phosphorylated src family kinase y416 (Cell Signaling Technology Inc)
Cell Signaling Technology Inc is a verified supplier 
Cell Signaling Technology Inc manufactures this product 
94
Structured Review
Cell Signaling Technology Inc
phosphorylated src family kinase y416
Phosphorylated Src Family Kinase Y416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+src+y416/Phospho-Src+Family+(Tyr416)+Rabbit+mAb/pm40739316-162-42-48
Average 94 stars, based on 68 article reviews
Phosphorylated Src Family Kinase Y416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphorylated+src+y416/Phospho-Src+Family+(Tyr416)+Rabbit+mAb/pm40739316-162-42-48
Average 94 stars, based on 68 article reviews
phosphorylated src family kinase y416 - by Bioz Stars,
2026-10
94/100 stars
Images
Related Articles
Staining:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. BrdU Incorporation Assay:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Quantitative RT-PCR:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Phospho-proteomics:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Western Blot:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Control:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Transfection:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Activity Assay:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Dominant Negative Mutation:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Construct:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Two Tailed Test:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Microarray:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Biomarker Discovery:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Expressing:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Activation Assay:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. Immunoprecipitation:Article Title: Chiral diaryl macrocycles and uses thereof Article Snippet: Immunblotting for Cellular Kinase Phosphorylation Assays NSCLC cell line H2228 (harboring endogenous EML4-ALK fusion gene), HCC78 cells (harboring endogenous SLC34A2-ROS1 fusion gene), Karpas-299 cells (harboring endogenous NPM-ALK fusion gene) or SET-2 (harboring endogenous JAK2V617F activating mutation) were cultured in RPMI medium, and KM12 (harboring endogenous TPM3-TRKA fusion gene) cell line was cultured in DMEM medium, both supplemented with 10% fetal bovine serum and 100 U/mL of penicillin/streptomycin. Article Title: A unique role for clathrin light chain A in cell spreading and migration Article Snippet: Data from 5–7 plating experiments were summarized together, and normalized to the mean area of control cells plated for 30 min. For live-cell phase-contrast imaging, siRNA-transfected cells were serum starved in suspension for 1 h, then plated on 35 mm glass-bottom dishes (Mattek) treated with collagen IV. |
![FIGURE 2 Dose-dependent effects of chronic administration of clozapine on expression of pannexin1 and <t>phosphorylated</t> Src in the mPFC. Rats were chronically administered with clozapine (0, 5 and 10 mg kg1 day1 for 28 days). In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH (a) and phosphorylated-Src (pSrc) per Src (b). *P < 0.05: relative to control (clozapine free) by one-way ANOVA with Scheffe's post hoc test. F-values of the chronic clozapine administration on the expression of pannexin1 and pSrc in the rat frontal cortex using one-way ANOVA were [F(3,15) = 17.3 (P < 0.01)] and [F(3,15) = 18.0 (P < 0.01)], respectively. The lower panels indicate pseudo-gel images of capillary immunoblotting.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_3081/pm40533081/pm40533081__page8_image1.jpg)
![FIGURE 6 Concentration-dependent effects of chronic administration of clozapine on expression of pannexin1 and phosphorylated Src in cultured astrocytes. (a and b) Concentration-dependent effects of chronic exposure to clozapine (3 and 10 μM for 28 days) on expression of pannexin1 and phosphorylated Src in the astroglial plasma membrane fraction, respectively. In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH or phosphorylated Src (pSrc) per Src. *P < 0.05: relative to control (clozapine free) by one- way ANOVA with Scheffe's post hoc test. F-values of the concentration-dependent effects of chronic clozapine administration on the expression of pannexin1 and pSrc using one-way ANOVA were [F(2,15) = 21.3 (P < 0.01)] and [F(2,15) = 14.9 (P < 0.01)], respectively. The lower panels indicate pseudo-gel images of capillary immunoblotting.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_3081/pm40533081/pm40533081__page12_image1.jpg)
![FIGURE 7 Interaction among clozapine and inhibitors of Src and III-mGluR on the expression of pannexin1 and phosphorylated Src in the cultured astrocytes. (a) Interaction between 10 μM PP2 (Src inhibitor) and 10 μM clozapine on pannexin1 expression in the astroglial plasma membrane. (b) Interaction between 100 μM CPPG (III-mGluR inhibitor) and 10 μM clozapine on phosphorylated-Src expression in the astroglial plasma membrane. In the upper histograms, ordinate: mean ± SD (n = 6) of the relative levels of pannexin1 (PANX) per GAPDH or phosphorylated Src (pSrc) per Src. *P < 0.05: relative to control (clozapine free) by two-way ANOVA with Scheffe's post hoc test. @: P < 0.05: relative to 10 μM clozapine exposure by two-way ANOVA with Scheffe's post hoc test. F-values of the interaction between 10 μM PP2 and chronic exposure to 10 μM clozapine on the expression of pannexin1 using two-way ANOVA were [Fclozapine(1,20) = 55.5 (P < 0.01), FPP2(1,20) = 0.2 (P > 0.05), Fclozapine*PP2(1,20) = 1.1 (P > 0.05)]. F- values of the interaction between 100 μM CPPG and chronic exposure to 10 μM clozapine on the expression of pSrc using two-way ANOVA were [Fclozapine(1,20) = 56.0 (P < 0.01), FCPPG(1,20) = 27.7 (P < 0.01), Fclozapine*CPPG(1,20) = 0.4 (P > 0.05)]. The lower panels indicate pseudo-gel images of capillary immunoblotting.](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_3081/pm40533081/pm40533081__page13_image1.jpg)
![FIGURE 9 Effects of chronic administration of L-BAIBA on Src signalling in the cultured astrocytes. (a and b) Interaction among chronic exposure to 30 μM L-BAIBA for 28 days and inhibitors of III-mGluR (100 μM CPPG) and NMDAR (1 μM MK801), respectively. In the upper side histogram, ordinate: mean ± SD (n = 6) of the relative levels of phosphorylated-Src (pSrc) per Src. *P < 0.05: relative to control (L-BAIBA free) by two-way ANOVA with Scheffe's post hoc test. @: P < 0.05: relative to 30 μM L-BAIBA exposure alone. F-values of the interaction between 100 μM CPPG and chronic exposure to 30 μM L-BAIBA on the expression of pSrc using two-way ANOVA were [FL-BAIBA(1,20) = 27.6 (P < 0.01), FCPPG(1,20) = 59.3 (P < 0.01), FL-BAIBA*CPPG(1,20) = 2.7 (P > 0.05)]. F-values of the interaction between 1 μM MK801 and chronic exposure to 30 μM L-BAIBA on the expression of pSrc using two-way ANOVA were [FL-BAIBA(1,20) = 46.3 (P < 0.01), FMK801(1,20) = 1.0 (P > 0.05), FL-](https://pub-med-unpaywalled-images-cdn.bioz.com/pub_med_ids_ending_with_3081/pm40533081/pm40533081__page14_image1.jpg)
